News

Latest study from the lab available in biorxiv

https://www.biorxiv.org/content/early/2017/12/05/229534.article-metrics

ABSTRACT
Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study how sarcomeres are built, we performed transcriptome sequencing of developing Drosophila flight muscles and identified 40 distinct expression profile clusters. Strikingly, two clusters are strongly enriched for sarcomeric components. Temporal gene expression together with detailed morphological analysis enabled us to define two distinct phases of sarcomere development, which both require the transcriptional regulator Spalt major. During the sarcomere formation phase, 1.8 micrometer long immature sarcomeres assemble myofibrils that spontaneously contract. During the sarcomere maturation phase, these sarcomeres grow to their final 3.2 micrometer length and 1.5 micrometer diameter and acquire stretch-sensitivity. Interestingly, the final number of myofibrils per flight muscle fiber is determined at the onset of the first phase. Together, this defines a biphasic mode of sarcomere and myofibril morphogenesis – a new concept that may also apply to vertebrate muscle or heart development.

 

 postdoc position in the Schnorrer lab

You:
You are curiosity-driven, fascinated by dynamic processes;
You are experienced in live imaging (FRAP, FLIM, laser-cutting);
You enjoy independent scientific planning;

We:
We are located at Institute of Developmental Biology of Marseille (IBDM), an interdisciplinary research center with a strong focus on quantitative in vivo biology and biophysics;
We study the morphogenesis of muscle with a particular interest in the contractile apparatus, sarcomeres and myofibrils;
We combine systematic fly genetics with in vivo imaging;
http://muscledynamics.org
http://www.ibdm.univ-mrs.fr/equipe/muscle-dynamics

Goal:
This project aims to understand the dynamics of sarcomere assembly during muscle development;
We know that early sarcomeres are irregular structures that mature within 2 days to pseudo-crystalline molecular machines;
We have generated a collection of endogenously tagged sarcomeric proteins, whose differential recruitment and turn-over should inform us about the assembly dynamics of these machines;

Please send your application, including a motivation letter, your CV, a description of research interests, and contact information for two references to:
frank.schnorrer@univ-amu.fr

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 PhD position in the Schnorrer lab

Biomechanics of Sarcomeres

You:
You are a curiosity-driven young scientist, fascinated by nature;
You enjoy pursuing a challenging, highly innovative project;
You like working with the Drosophila model;

We:
We are located at Institute of Developmental Biology of Marseille (IBDM), an interdisciplinary research center with a strong focus on quantitative in vivo biology and biophysics;
We study the morphogenesis of muscle with a particular interest in the contractile apparatus, sarcomeres and myofibrils;
We combine systematic fly genetics with in vivo imaging;
http://muscledynamics.org
http://www.ibdm.univ-mrs.fr/equipe/muscle-dynamics

Goal:
This project aims to manipulate the mechanical properties of sarcomeric components, in particular of the gigantic spring domain protein titin and observe the phenotypic consequences;
This will apply innovative CRISPR-based genome modification strategies and super-resolution imaging;

Please send your application, including a motivation letter, your CV, a description of research interests, and contact information for two references to:
frank.schnorrer@univ-amu.fr

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