PhD Student
devampurohit9 [at] gmail.com
devam . purohit [at] univ-amu.fr
I completed my Integrated master’s in Cell and Molecular biology from The Maharaja Sayajirao University, Baroda. I then worked as a project fellow in the lab of Prof. K. VijayRaghavan (NCBS, Bangalore), in collaboration with Prof. Mani Ramaswami (Trinity College, Dublin). I worked on understanding the transcriptional consequences of stress response in Drosophila. I also established an in vivo genetic muscle degeneration model in Drosophila. My experience led me to pursue a multidisciplinary PhD at CENTURI where I’m aiming to understand the role of titin spring domain in muscle elasticity in the labs of Dr. Frank Schnorrer (IBDM) and Dr. Felix Rico (LAI).
Educational Background
| 2021 – Present | PhD in Developmental Biology |
| 2013-2018 | The Maharaja Sayajirao University of Baroda Integrated. M.Sc. in Cell and Molecular Biology |
Research Experience
| 2021 | PhD Student – Turing Centre for Living Systems (CENTURI) Supervisor: Dr. Frank Schnorrer (IBDM) and Dr. Felix Rico (LAI) Project: “The muscle spring: quantifying and manipulating the molecular elasticity of muscle in vitro and in vivo” |
| 2018 | Project Fellow – National Centre for Biological Sciences (NCBS), Bangalore Supervisor: Prof. K. VijayRaghavan and Prof. Mani Ramaswami Duration: 2018 – 2020 Project: “Elucidating role of transcriptional mechanism in adaptation to stress” Project: “Heterogeneous distribution of mRNAs within flight muscle fibres” |
| 2017 | M.Sc. dissertation: National Centre for Biological Sciences (NCBS), Bangalore Duration: 6 months Supervisor: Prof. K. VijayRaghavan and Prof. Mani Ramaswami Project: “Molecular Characterization of Ataxin-2 Stress Granules” Project: “Inducing stress granules in vivo” |
| 2016 | Summer training: Indian Institute of Science Education and Research (IISER), Pune. Duration: 2 months Supervisor: Prof. L.S Shashidhara Project: “Characterization of GRAF as tumour suppressor” |
| 2015 | Summer training: National Institute for Research in Tuberculosis, Chennai Duration: 1.5 months Supervisor: Dr. Soumya Swaminathan Project: lab visitation to HIV/AIDS, Bacteriology, ICER and Immunology departments. |
| 2014 | Summer training: The Maharaja Sayajirao University of Baroda Duration: 3 weeks. Project: One-week training in Botany, Zoology and Chemistry department. |
Conferences and Workshops
2020 Presented poster at “Circle of Life – Annual Talks” at NCBS, Bangalore,
“The cellular response to Oxidative Stress”
2020 Presented at Max Planck Institute – IEM, Freiburg for Final PhD round.
2019 Hands-on-Workshop on NGS data analysis at IBAB, Bangalore
2019 Presented poster at “Language of Biology – Annual Talks” at NCBS, Bangalore,
“Assembly and Clearance of Stress Granules – intracellular foci implicated in ALS and Ataxia”
2018 ADBS-IBAB Whole Genome Sequencing Workshop at NCBS, Bangalore.
2018 International Congress of Cell Biology at CCMB, Hyderabad.
2016 National Symposium on “Omics, Structural Basis of Diseases” at MSU, Vadodara
2014 National Symposium on “Emerging Trends in Biochemical Sciences” at MSU, Vadodara
Technical skills
Molecular Biology: Cloning, PCR, Gel Electrophoresis, Transformation, Transfection, SDS-PAGE and western blotting, RNA isolation
Transcriptomics: Mapping: STAR, HISAT and Tophat (Linux). Read counting: HTSeq.
Differential gene expression analysis: DESeq2 and edgeR. Differential exon usage analysis: DEXSeq.
RNA-Seq data analysis and visualization using R and Cytoscape.
Microscopy: Immunohistochemistry, confocal microscopy and image analysis using ImageJ (Fiji).
Insect cell culture (S2R+), D. melanogaster handling
Publications
- Parekh, A., Chakraborty, K., Purohit, D.J., Pasha, S.N., Sowdhamini, R., VijayRaghavan, K., and Chaturvedi, D. (2020). Heterogeneous distribution of mRNAs within flight muscle fibers, and implications for function. bioRxiv. Current Status: Under Review
- Singh, A., Kandi, A. R., Jayaprakashappa, D., Thuery, G., Purohit, D. J., Huelsmeier, J., . . . Bakthavachalu, B. (2021). The transcriptional response to oxidative stress is independent of stress-granule formation. bioRxiv. Current Status: Under Review